parameter single cell analysis has driven deep understanding of immune
processes. Using a next-generation
single-cell “mass cytometry” platform we quantify surface and cytokine or drug
responsive indices of kinase target with 45 or more parameter analyses (e.g. 45
antibodies, viability, nucleic acid content, and relative cell size).
Similarly, we have developed two advanced technologies termed MIBI and CODEX
that enable deep phenotyping of solid tissue in both fresh frozen and FFPE formats
(50 – 100 markers). Collectively, the
systems allows for subcellular analysis from the 70nm resolution scale to whole
tissue in 3D.
I will present
evidence of deep internal order in immune functionality demonstrating that
differentiation and immune activities have evolved with a definable
“shape”. Further, specific cellular neighborhoods of immune cells are now
definable with unique abilities to affect cellular phenotypes—and these
neighborhoods alter in various cancer disease states. In addition to cancer, these shapes and
neighborhoods are altered during immune action and “imprinted” during, and
after, pathogen attack, traumatic injury, or auto-immune disease. Hierarchies of functionally defined
trans-cellular modules are observed that can be used for mechanistic and
clinical insights in cancer and immune therapies.